Work

Preparation of Vaccine from Biofilm Proteins of Staphylococcus aureus and Incorporate it in Liposomes for Induction of CXCL-5 Production in Albino Rats

Year: 2024

Type: article

Abstract: In this study biofilm matrix proteins (BMP) were isolated from bacterial biofilm and incorporated in a delivery system (liposome) to stimulate an immune response against virulent pathogens. The specimens were cultured and identified depending on morphological and biochemical tests . Around 67.74% of isolates were found to be biofilm formers. The immunomodulatory activity revealed that BMP-liposome vaccine and BMP vaccine significantly increased chemokine CXCL5 levels in sera of rats in first and second doses. The concentration of total WBCs count, neutrophils, and lymphocytes also increased. In rats groups that had received BMP-liposome vaccine, the challenge test revealed high immunogenicity and effective protection. It can, therefore, be concluded that BMP-liposome are a promising nanomedicine for regulating biofilm hyper-reaction. They may, however, cause tissue inflammation. On the other hand, it appeared to be helpful in activating the immune response at moderate levels, allowing infection to be eradicated without tissue damage.

Source: Iraqi Journal of Science

DOI: 10.24996/ijs.2024.65.8.5

Authors: Zahraa Abdulrazaq AlKhfaji, Mayyada Farhan Darweesh, Ali Fattah Al-Musawi

Institutions: University of Kerbala, Al-Zahraa University for Women, University of Kufa, University of Alkafeel, Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4402068558

FWCI:

Cites: 23

Cited by:

Related to: 10