Work

Optimization of the formulation of Tubiechong gel plaster and its effect on fracture healing by promoting angiogenesis in a rat model

Year: 2024

Type: article

Abstract: Background and aim: Transdermal drug delivery has been used in traditional Chinese medicine for thousands of years and is worth further developing. The purpose of this study was to investigate the preparation method of Tubiechong gel plaster (TGP) and evaluate its activity to promote fracture healing. Experimental procedure: permeability of the plaster was studied by Franz diffusion cell method. A modified Einhorn method was used to model rat tibia fractures. The bone healing process was monitored by radiography. Bone development and angiogenesis were assessed by Safranin O Fast Green staining and CD31 immunohistochemistry, respectively. Serum bone metabolites and VEGF levels were determined by ELISA method. Results and conclusion: The optimal dose of azone for TGP is 2 %. The transdermal penetration equation for this plaster is consistent with the Ritger-Peppas equation. The prepared plaster was stable for at least 3 months. X-ray images showed that the fracture healing was promoted by TGP in a dose-related form at all periods. Serum BALP, OC, CTX-I, and VEGF levels also indicated better fracture healing after treatment. TGP significantly stimulated angiogenesis and bone growth at the fracture site on days 7,14 and 21, and the effect of promoting bone growth was shown on the third day. These data prove that the prepared Tubiechong gel plasters can promote fracture healing by promoting angiogenesis.

Source: Journal of Traditional and Complementary Medicine

DOI: 10.1016/j.jtcme.2024.08.009