Work

Strategy in Synthesizing Longer-Chain Levan-Type Fructooligosaccharides by Selective Dextran Macromolecular Cross-Linked Bacillus lehensis G1 Endolevanase Aggregate Immobilization

Year: 2024

Type: article

Abstract: The formation of cross-linked enzyme aggregates (CLEAs) using macromolecular cross-linkers improves substrate accessibility and enhances enzyme retention. However, there have been few studies exploring the use of macromolecular cross-linkers due to the challenges related to cross-linker screening. In compliance with our previous computational and experimental screening, dextran is the optimal macromolecular cross-linker to develop CLEAs of endolevanase from Bacillus lehensis G1 (rlevblg1-dex-CLEA) for levan-type-fructooligosaccharides (L-FOS) production. In this study, rlevblg1-dex-CLEAs was optimized, and the activity recovery continued to increase and reached 90.5%. Subsequently, the rlevblg1-dex-CLEAs were characterized and they displayed higher thermal stability after 1 h of incubation in comparison to the free enzyme. Moreover, the rlevblg1-dex-CLEAs were reusable for five cycles and exhibited greater storage stability over 180 days at 4 °C (60.9%) than that of free rlevblg1. In addition, the rlevblg1-dex-CLEAs demonstrated similar catalytic efficiency as the free enzyme and generated a substantial amount of L-FOS with a longer degree of polymerization, which is more beneficial for industrial use.

Source: Catalysts

DOI: 10.3390/catal14090584

Authors: Hotaf Hassan Makki, Nardiah Rizwana Jaafar, Nashriq Jailani, Abdullah A. Alqasem, Zaidah Rahmat, Rosli Md. Illias

Institutions: Ministry of Education, University of Technology Malaysia, Prince Sattam Bin Abdulaziz University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,069

Funder: Universiti Teknologi Malaysia