Temporal and spatial resolution of magnetosome degradation at the subcellular level in a 3D lung carcinoma model
Year: 2024
Type: article
Abstract: Magnetic nanoparticles offer many exciting possibilities in biomedicine, from cell imaging to cancer treatment. One of the currently researched nanoparticles are magnetosomes, magnetite nanoparticles of high chemical purity synthesized by magnetotactic bacteria. Despite their therapeutic potential, very little is known about their degradation in human cells, and even less so of their degradation within tumours. In an effort to explore the potential of magnetosomes for cancer treatment, we have explored their degradation process in a 3D human lung carcinoma model at the subcellular level and with nanometre scale resolution. We have used state of the art hard X-ray probes (nano-XANES and nano-XRF), which allow for identification of distinct iron phases in each region of the cell. Our results reveal the progression of magnetite oxidation to maghemite within magnetosomes, and the biosynthesis of magnetite and ferrihydrite by ferritin.
Source: Journal of Nanobiotechnology
Institutions: University of the Basque Country, Centre National de la Recherche Scientifique, Aix-Marseille Université, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biosciences et Biotechnologies, Biologie Végétale et de Microbiologie Environnementales, Institut de Biosciences et biotechnologies d'Aix-Marseille, University of Pannonia, Brookhaven National Laboratory, National Synchrotron Light Source II
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,490
Funders: U.S. Department of Energy, Ministerio de Ciencia, Innovación y Universidades, European Synchrotron Radiation Facility, Eusko Jaurlaritza, Euskal Herriko Unibertsitatea, Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, Office of Science, European Regional Development Fund, Agencia Estatal de Investigación, National Research, Development and Innovation Office, Brookhaven National Laboratory
Awards: G1054051337, G1105844351, G115316231, G115937378, G1175115578, G1220307624, G1285306620, G1338257350, G1376759263, G1868695577, G2089874362, G2262748287, G241279073, G300979063, G3262563802, G3428307696, G34346518, G3480869486, G357145406, G3850097093, G4033406476, G410571670, G4181270102, G425668148, G451917667, G4568341554, G491712853, G4972998915, G5080475149, G5417557083, G5539308778, G5633341255, G5635532810, G5765906131, G5943232429, G5967599077, G624877701, G6731524417, G6733788163, G7084143925, G7463374569, G7805626310, G7863371703, G8497050386, G929308097
OpenAlex ID: W4402130718