Work

Sequential Injection Analysis Method for the Determination of Glutathione in Pharmaceuticals

Year: 2024

Type: article

Abstract: A sequential injection analysis method for the determination of glutathione (GSH) in pharmaceuticals has been developed. It is based on the reduction of the Cu(II)-neocuproine complex by GSH and the formation of an orange-yellow colored Cu(I)-neocuproine complex with maximum absorbance at 458 nm. Under optimal conditions the method is characterized by a linear calibration range of 6.0 × 10−7–8.0 × 10−5 mol L−1 (Amax = 3270 CGSH − 0.0010; R2 = 0.9983), limit of detection of 2.0 × 10−7 mol L−1, limit of quantification of 6.7 × 10−7 mol L−1, repeatability (expressed as relative standard deviation) of 3.8%, and sampling rate of 60 h−1. The newly developed method has been successfully applied to the determination of GSH in pharmaceutical samples with no statistically significant difference between the results obtained and those produced by the standard Pharmacopoeia method.

Source: Sensors

DOI: 10.3390/s24175677

Authors: Maja Biocic, Tomislav Kraljević, Tony G. Spassov, Lea Kukoc-Modun, Spas D. Kolev

Institutions: University of Split, University of Mostar, Sofia University "St. Kliment Ohridski", The University of Melbourne

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,955

Funder: European Commission