Development Of a Recombinant Lactobacillus Salivarius for Prophylactic Applications Against Mycobacterium Avium Subspecies Paratuberculosis and Johne's Disease
Year: 2013
Type: dissertation
Abstract: Current whole-cell vaccines against Mycobacterium avium subsp. paratuberculosis, the etiological agent of Johne’s disease, are unable to provide complete protection against infection. Moreover, such whole-cell formulations interfere with diagnostic tests for bovine tuberculosis (Mycobacterium bovis). The development of subunit vaccines, those which are preferentially based on one, or a select few, immunodominant protective antigens is considered as the realistic next step in overcoming these issues. The (MAP) K-10 genome is a single circular chromosome of 4,829,781 base pairs and encodes 4,350 predicted ORFs. In order to identify MAP specific proteins for subsequent incorporation within a mucosal based vaccine development, a subtractive sequence-based comparison was conducted using the entire MAP K-10 genome against a number of other completed Mycobacterial genomes. A bank of five candidate antigens including MAP specific, membrane associated and virulence related proteins has been identified. The expression of these antigenic candidates within a Lactobacillus salivarius delivery host will require further investigation.
DOI: 10.34719/cvsd1135
Author: Christopher Johnston
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4402168782