Figure S5 from Blockade of IL1β and PD1 with Combination Chemotherapy Reduces Systemic Myeloid Suppression in Metastatic Pancreatic Cancer with Heterogeneous Effects in the Tumor
Year: 2024
Type: other
Abstract: Supplemental Figure 5: Addition of TNFa ameliorates myeloid suppression in vitro. (A) Histogram of HLA-DR mean fluorescence intensity of CD33+ SSCA high cells from monocytes cocultured for a week with 75 pg/ML M-CSF with the addition of 10 ng/mL each GM-CSF/ IL-6 or 10ng/mL IL-1b with or without an IL-1b blocking antibody. (B) Induced MoMDSCs from panel A were then cultured with CFSE-labeled T cells for 3 days coculture at a 1:1 ratio, and T cell proliferation was assessed by flow cytometry. Proliferation indices were calculated. (C) MoMDSCs induced from monocytes cultured for 7 days with the indicated cytokines were then cultured with CFSE-labeled T cells for 3 days coculture at a 1:1 ratio, and T cell proliferation was assessed by flow cytometry. Representative histograms are shown. (D) Proliferation indices from the data shown in C were calculated.
Authors: Paul E. Oberstein, Andressa Dias Costa, Emily A. Kawaler, Victoire Cardot-Ruffino, Osama E. Rahma, Nina Beri, Harshabad Singh, Thomas A. Abrams, Leah H. Biller, James M. Cleary, Peter Enzinger, Brandon M. Huffman, Nadine J. McCleary, Kimberly J. Perez, Douglas A. Rubinson, Benjamin L. Schlechter, Rishi Surana, Matthew B. Yurgelun, S. Jennifer Wang, Joshua Remland, Lauren K. Brais, Naima Bollenrucher, Eugena Chang, Lestat R. Ali, Patrick J. Lenehan, Igor Dolgalev, Gregor Werba, Cibelle Lima, C. Elizabeth Keheler, Keri M. Sullivan, Michael Dougan, Cristina Hajdu, Maya Dajee, Marc R. Pelletier, Saloney Nazeer, Matthew Squires, Dafna Bar-Sagi, Brian M. Wolpin, Jonathan A. Nowak, Diane M. Simeone, Stephanie K. Dougan
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4402201055