Work

Intact-skull cranial windows for widefield optical imaging in juvenile mice: complications and consequences

Year: 2024

Type: article

Abstract: Functional neuroimaging with widefield optical imaging (WOI) is potentially useful for studying developmental disorders in juvenile mice. However, WOI requires an intact-skull cranial window, and the effects of such windows on young mice are unknown. We performed intact-skull cranial window placement on mice as young as P7 to study the effects of chronic placement. Cranial windows placed at young ages (P7 and P10) were not longitudinally stable, resulting in significant attrition. Windows placed at ages P14 or less resulted in significant impairment to skull growth, which in turn caused artifacts in resting-state functional connectivity analysis. Longitudinal cranial windows should likely be avoided under P30.

Source: Journal of the Optical Society of America A

DOI: 10.1364/josaa.531568

Authors: Temilola E. Adepoju, Hayden B. Fisher, Chloe Winston, Brian R. White

Institutions: Children's Hospital of Philadelphia, University of Pennsylvania

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Neurological Disorders and Stroke