Evaluation of amlodipine against strains of Candida spp. in planktonic cells, developing biofilms and mature biofilms
Year: 2024
Type: article
Abstract: Aim: To evaluate the antifungal activity of amlodipine against strains of Candida spp. and to its possible mechanism of action.Methods: Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.Results: Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.Conclusion: Amlodipine had good antifungal activity against planktonic cells and biofilms of Candida spp., by leading the cells to apoptosis.
Source: Future Microbiology
Authors: Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Fátima Daiana Dias Barroso, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Érica Rayanne Motta da Costa, Leilson Carvalho de Oliveira, Amanda Dias Barbosa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva
Institutions: Universidade Federal do Ceará, Unichristus
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4402275375