Work

Simultaneous determination of cucurbitacins B, D, and E in Cucurbita seed oils by HPLC-DAD

Year: 2024

Type: article

Abstract: Cucurbitacins are triterpene bioactive constituents of natural products, particularly in the Cucurbitaceae plant family. The presence of cucurbitacins in seeds of the Cucurbita genus (pumpkin) has been only little studied. In this work, the content of cucurbitacins B, D, and E in seed oils from three cucurbits (Cucurbita moschata Duch, Cucurbita pepo Linn, and Cucurbita maxima Linn) was studied. An analytical method based on HPLC-DAD for the detection and quantification of these three cucurbitacins in seed oils was developed and validated according to ICH guidelines. The method showed good linearity, accuracy, and precision for the simultaneous quantification of cucurbitacins B, D, and E using C.moschata seed oil as a reference. When applied to C.pepo and C.maxima seed oils, cucurbitacin B and D were quantified but to a lesser extent. This is the first report of a simple, repeatable, and reproducible analytical tool to identify cucurbitacins in oilseeds from Cucurbita spp.

Source: Natural Product Research

DOI: 10.1080/14786419.2024.2394097

Authors: Elizabeth Goya-Jorge, María Elisa Jorge-Rodríguez, Yanelis Saucedo-Hernández

Institutions: North Carolina State University, Universidad Central "Marta Abreu" de las Villas (UCLV)

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Zero hunger

Open Access status: green

Funder: Vlaamse Interuniversitaire Raad