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Protocol for preparing cyclic-phospholipid decanoate and glyceryl-didecanoate-phosphate-containing vesicles

Year: 2024
Type: article
Abstract: Cyclic-phospholipids-based vesicles can play a role in facilitating the chemical evolution of protocells from the structurally simple to the functionally more complex form. Here, we present a protocol for preparing decanoic acid-derived cyclic phospholipid and glyceryl-diester phosphate-containing vesicles. We describe steps for sample preparation, equilibration, and image acquisition using confocal microscopy. This protocol has the potential for preparing a wide variety of these phospholipid-based artificial cell constructs. For complete details on the use and execution of this protocol, please refer to Pulletikurti et al. 1 • Steps for preparing cyclic-phospholipid-based vesicles • Instructions for acquisition of images and analysis of vesicles from the samples • Troubleshooting steps and guidance on sample preparation and equilibration Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cyclic-phospholipids-based vesicles can play a role in facilitating the chemical evolution of protocells from the structurally simple to the functionally more complex form. Here, we present a protocol for preparing decanoic acid-derived cyclic phospholipid and glyceryl-diester phosphate-containing vesicles. We describe steps for sample preparation, equilibration, and image acquisition using confocal microscopy. This protocol has the potential for preparing a wide variety of these phospholipid-based artificial cell constructs.
DOI: 10.1016/j.xpro.2024.103283
Language: English
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4402285012
FWCI: 0.2
Cites: 8
Cited by: 1
Related to: 10