Work

Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging

Year: 2024

Type: article

Abstract: Membrane proteins often need to be inserted into or attached to the cell membrane to perform their functions. Understanding their transmembrane topology and conformational dynamics during insertion is crucial for elucidating their roles. However, it remains challenging to monitor nanoscale changes in the insertion depth of individual proteins in membranes. Here, we introduce two single-molecule imaging methods, SIFA and LipoFRET, designed for in vitro observation of the nanoscale architecture of membrane proteins within membranes. These methods have demonstrated their efficacy in studying biomolecules interacting with bio-membranes with sub-nanometer precision.

Source: Biophysics Reports

DOI: 10.52601/bpr.2024.240024

Authors: Chenguang Yang, Dongfei Ma, Shuxin Hu, Ming Li, Ying Lu

Institutions: Chinese Academy of Sciences, Institute of Physics, University of Chinese Academy of Sciences, Songshan Lake Materials Laboratory

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, Basic and Applied Basic Research Foundation of Guangdong Province

Awards: G4204445851, G5338286085