Work

Solvent optimization and fluorescein lasing for avidin biosensing

Year: 2024

Type: article

Abstract: We report pulsed laser emission using fluorescein combined with avidin as an active medium matrix in a Littrow cavity configuration. The study covers testing several solvents and a range of excitation energies, aiming to get the narrowest bandwidth for stable peak emission wavelength. Initially, for 4 mM of fluorescein in 1:1 mixture of methanol:NSS, and excited with 20.80 mJ of energy, a laser mission of 4.38 nm bandwidth is achieved. Then, different concentrations of thiolated avidin are mixed in the active medium, causing related red-shifts over the initial emission band. Substantial spectral changes are observed down to 0.3 μM of avidin, making apparent the stability conditions for this sort of laser system to work out as a biosensor.

Source: Materials Letters

DOI: 10.1016/j.matlet.2024.137377

Authors: E. Mendicuti, C. García-Segundo, M.B. de la Mora, Mayo Villagrán-Muniz

Institution: Universidad Nacional Autónoma de México

Language: English

Topic: Solid State Laser Technologies

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $2,540

Funders: Consejo Nacional de Ciencia y Tecnología, Universidad Nacional Autónoma de México, Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México