Work

Preparation of photo-controlled release ROS-responsive Ce6/elemene co-loaded liposomes and study on the effect on enhancing apoptosis of NMIBC

Year: 2024

Type: article

Abstract: At present, chemotherapy combined with photodynamic therapy is exerting satisfactory therapeutic effects in the treatment of tumors. Chlorin e6 (Ce6) is a photosensitizer with high efficiency and low dark toxicity. At the same time, elemene (ELE) contains high-efficiency and low-toxicity anti-cancer active ingredients, which can effectively penetrate tumor tissue and inhibit its recovery and proliferation. Due to the poor water solubility of these two drugs, we prepared ELE/Ce6 co-loaded liposomes (Lipo-ELE/Ce6) to improve their water solubility, thereby enhancing the anti-tumor effect. The characterization of Lipo-ELE/Ce6 showed that Lipo-ELE/Ce6 had suitable encapsulation efficiency (EE), particle size, polydispersity (PDI), zeta potential, and good photo-controlled release properties. In vitro, Lipo-ELE/Ce6 effectively inhibited the growth of T24 cells and induced apoptosis, and more importantly, in vivo experiments showed that Lipo-ELE/Ce6 had significant anti-tumor effects, which was significantly better than free drugs. The above results suggest that Lipo-ELE/Ce6 can significantly enhance the induction of apoptosis of non-muscle invasive bladder cancer (NMIBC) by light-controlled release and ROS response. Schematic illustration of Lipo-ELE/Ce6 for combined photo-chemotherapy. (A) Construction of Lipo-ELE/Ce6; (B) Effect of Lipo-ELE/Ce6 + Laser on enhancing apoptosis of NMIBC. • Photorelease ROS-responsive co-loaded ELE/Ce6 liposomes constructed. • Lipo-ELE/Ce6 synergises Ce6 and ELE through irradiation. • Solving the problem of Ce6's high hydrophobicity and lack of efficacy when used alone. • Elucidation of elemene combined with PDT to achieve synergistic anti-tumour effects. • Providing a combined Chinese medicine programme for the clinical treatment of NMIBC.

Source: Biomedicine & Pharmacotherapy

DOI: 10.1016/j.biopha.2024.117398