Year: 2024
Type: article
Abstract: Previously established World Health Organization (WHO) International Standards (IS) for anti-HPV16 and HPV18 antibodies are used to harmonize results across human papillomavirus (HPV) serology assays. Here, we present an international collaborative study to establish ISs for antibodies against HPV6 (NIBSC code 19/298), HPV11 (20/174), HPV31 (20/176), HPV33 (19/290), HPV45 (20/178), HPV52 (19/296) and HPV58 (19/300). The candidate standards were prepared using sera from naturally infected individuals. Each candidate was shown to be monospecific for reactivity against its indicated HPV type except for the HPV11 candidate, which was also reactive against other types. Expression of antibody levels relative to the relevant candidate IS reduced inter-laboratory variation allowing greater comparability between laboratories. Based on these results, the WHO Expert Committee on Biological Standardization established each of the 7 candidates as the 1st IS for antiserum to its indicated HPV type for use in the standardization of HPV pseudovirion-based neutralization and antibody-binding assays.
Source: npj Vaccines
Authors: Troy J. Kemp, Gitika Panicker, Carina Eklund, Jianhui Nie, Youchun Wang, Simon Beddows, Peter Rigsby, Weijin Huang, Joakim Dillner, Elizabeth R. Unger, Ligia A. Pinto, Dianna E. Wilkinson, the collaborative study participants, Paul Licciardi, Zheng Quan Toh, Martin Müller, T. M. Chozhavel Rajanathan, Shaowei Li, Ningshao Xia, Ge Liu, Chenliang Zhou, Lingyun Zhou, Nan Xu, Kavita Panwar, Denise Galloway, Jody Carter
Institutions: Frederick National Laboratory for Cancer Research, Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Karolinska University Hospital, Karolinska Institutet, National Institutes for Food and Drug Control, UK Health Security Agency, Medicines and Healthcare products Regulatory Agency, Murdoch Children's Research Institute, German Cancer Research Center, Zydus Lifesciences Limited (India), Xiamen University, Fred Hutch Cancer Center
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,690
OpenAlex ID: W4402399377