Unlocking the in vitro neuroprotection of sloe residues phenolic extracts by bioanalytical and chemometric strategies
Year: 2024
Type: article
Abstract: Wild fruits, particularly the underutilized sloe ( Prunus spinosa ), are gaining interest as natural antioxidants, with residues from liqueur production being a source of bioactive compounds. This study proposes a sustainable approach for valorizing sloe residues, seeds and skins, by employing an innovative green extraction method. HPLC-ESI-QTOF and spectrophotometric techniques were used to explore the phenolic profile, highlighting the predominance of quercetin, 2,3-dihydroxybenzoic and ferulic acids (9.7–57 μg·g −1 ). In addition, the presence of Cu, Zn and Ca was confirmed by atomic absorption spectroscopy. Simultaneously, their neuroprotective potential against Alzheimer's disease (AD) was studied by exploring the inhibition of beta-amyloid aggregation and oxidative stress cytoprotection in SH-SY5Y cell line, standing out 1 μg·g −1 and 10 μg·g −1 extracts of sloe skin. Phenolic composition was correlated with bioactivities by means of multivariate analysis. These results contributed to highlight the potential of this bio-residue as a neuroprotective agent against AD in pharmaceutical and nutraceutical industries. • First-time evaluation of the neuroprotective effect of sloe ( Prunus spinosa ). • Sloe skin and seed extracts were rich in quercetin, dihydroxybenzoic and ferulic acid. • Sloe phenolic extract inhibited Aβ 42 aggregation involved in Alzheimer's disease (AD). • Sloe skin extract showed in vitro antioxidant effect over SH-SY5Y neuroblastoma cells. • Bioactivities and phenolic composition have been correlated by multivariate analysis.
Source: Food Chemistry
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,620
OpenAlex ID: W4402404845