Work

Adapting to the acidic environment of the NP: RADA16-PLGA (TGF-β3) induces chondrogenic differentiation of BMSCs

Year: 2024

Type: article

Abstract: Aim: RADA16-PLGA composite scaffolds constructed with simultaneous loading of BMSCs and TGF-β3 and explored their ability for chondrogenic differentiation in vitro.Methods: The performance of the composite scaffolds is assessed by rheometer assay, electron microscopic structural observation and ELISA release assay. The biosafety of the composite scaffolds is assessed by cytocompatibility assay and cell migration ability. The chondrogenic differentiation ability of composite scaffolds is evaluated by Alisin blue staining, PCR and immunofluorescence staining.Results: The composite scaffold has a good ECM-like structure, the ability to control the release of TGF-β3 and good biocompatibility. More importantly, the composite scaffolds can induce the differentiation of BMSCs to chondrocytes.Conclusion: Composite scaffolds are expected to enhance the endogenous NP repair process.

Source: Nanomedicine

DOI: 10.1080/17435889.2024.2372242

Authors: Xin Yao, Shaolong Li, Maoqiang Lin, Weiyuan Xu, Xiaobo Zhang, Haiyu Zhou

Institutions: Lanzhou University Second Hospital, Xi'an Honghui Hospital, Xi'an Jiaotong University

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Natural Science Foundation of Gansu Province