Year: 2024
Type: article
Abstract: Aim: This study aimed to investigate the impact of cytosine 5'-monophosphate (5'-CMP), guanosine 5'-monophosphate (5'-GMP), and uridine 5'-monophosphate (5'-UMP), along with their disodium salts, in combination with D-mannitol (D-M), on the conformation and fluorescence properties of interferon α-2b (IFN), and to determine the energy parameters governing these interactions.Methods: Spectroscopic techniques including time-resolved spectroscopy, circular dichroism, and fluorescence spectroscopy, along with isothermal nanocalorimetry, were employed.Results: The nucleotide monophosphates (5'-rN-MFs) in their acidic form, particularly when complexed with D-mannitol, exhibited stronger binding to interferon α-2b compared to their salt forms.Interaction between IFN α-2b and 5'-rNMFs, especially in the presence of D-mannitol, was found to be exothermic and induced changes in the conformational flexibility of IFN α-2b,
Source: Biopolymers and Cell
DOI: 10.7124/bc.000ab9
Authors: R. O. Nikolaiev, Z. Yu. Tkachuk
Institution: Institute of Molecular Biology and Genetics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4402418339