Work

Development of an immunochromatographic strip for the rapid detection of 11-dehydro-thromboxane B2 in human urine

Year: 2024

Type: article

Abstract: Urinary 11-dehydro-thromboxane B2 (11-DH-TXB2) is a diagnostic marker for evaluating the effect of aspirin treatment in patients with cardiovascular disease. Therefore, it is of great clinical significance to develop a rapid detection method for 11-DH-TXB2 in urine. In this study, we prepared an ultrasensitive monoclonal antibody (mAb) against 11-DH-TXB2. The mAb belongs to the IgG1 subtype with kappa light chains. In an indirect competitive ELISA , it had a half-maximal inhibitory concentration of 3.63 ng/mL and a high affinity of 5.60 × 10 9 L/mol for 11-DH-TXB2. We then developed an immunochromatographic strip for point-of-care testing (POCT) of 11-DH-TXB2 in human urine. The strip provides a cut-off value and limit of detection of 500 ng/mL and 31.2 ng/mL, respectively. The result is obtained within 15 min. In summary, the proposed strip is a portable POCT tool for monitoring 11-DH-TXB2 in human urine.

Source: Chinese Journal of Analytical Chemistry

DOI: 10.1016/j.cjac.2024.100436

Authors: Guangxing TAN, Xiaoqian JIANG, Chuanlai XU, Zhuyuan FANG

Institutions: Nanjing University of Chinese Medicine, Jiangnan University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Natural Science Foundation of China, National Outstanding Youth Science Fund Project of National Natural Science Foundation of China, National Key Research and Development Program of China