Year: 2024
Type: article
Abstract: Biocatalytic steroid hydroxylation processes are highly relevant for the pharmaceutical industry but are still limited by low specific activities and stabilities. For testosterone hydroxylation, high activities were achieved with recombinant Escherichia coli containing a highly active CYP450-BM3 variant and outer membrane porins. Enzyme instability and substrate mass transfer were identified as further limitations, for which biocatalyst and reaction engineering strategies are discussed.
Source: BIOspektrum
Authors: Carolin Bertelmann, Bruno Bühler
Institution: Helmholtz Centre for Environmental Research
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,090
OpenAlex ID: W4402461827