Work

Generation of a hiPSC line (TONGJIi001-A) from a 46,XX,ins(1;15)(p13.3; q22.31q26.1),inv(2)(p22.1p16.3),t(2;14)(q34;q12) infertility patient

Year: 2024

Type: article

Abstract: We have successfully derived a hiPSC line from PBMCs obtained from a 41-year-old infertile female. The patient's karyotype, as determined by Bionano OGM, revealed complex chromosomal rearrangements, including 46,XX,ins(1;15)(p13.3;q22.31q26.1),inv(2)(p22.1p16.3),t(2;14)(q34;q12). Specifically, the episomal plasmids encoding key reprogramming factors OCT4, sh-p53, SOX2, KLF4, L-MYC, and LIN28 were applied to generate the integration-free hiPSC line, which was designated as TONGJIi001-A. This line exhibits typical hiPSC morphology, expresses core pluripotency markers and presents the ability to differentiate into all three germ layers in vitro. Collectively, hiPSC TONGJIi001-A provides a valuable resource for investigating the mechanisms underlying chromosomal structural abnormalities associated with infertility.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2024.103561

Authors: Yunzhe Kang, Shanshan Liang, Shaorong Gao, Jiayu Chen

Institutions: Tongji University, Shanghai First Maternity and Infant Hospital

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,320