Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging
Year: 2024
Type: article
Abstract: This study introduces the time-gated analysis of room-temperature phosphorescence (RTP) for the in-situ analysis of the visible and spectral information of photons. Time-gated analysis is performed using a microscopic system consisting of a spectrometer, which is advantageous for in-situ analysis since it facilitates the real-time measurement of luminescence signal changes. An RTP material hybridized with a DNA aptamer that targets a specific protein enhances the intensity and lifetime of phosphorescence after selective recognition with the target protein. In addition, time-gated analysis allows for the millisecond-scale imaging of phosphorescence signals, excluding autofluorescence, and improves the signal-to-background ratio (SBR) through the accumulation of signals. While collecting the time-gated images and spectra of RTP and autofluorescent materials simultaneously, we develop a method for obtaining phosphorescence signals by means of selective exclusion of autofluorescence signals in simulated or real cell conditions. It is confirmed that the accumulated time-gated analysis can provide ample information about luminescence signals for bioimaging and biosensing applications. • The RTP materials are hybridized with DNA aptamer for bio-recognition avoiding autofluorescence with time-gated analyses. • A spectrometer is integrated with microscopy to obtain visible and spectral data, enhancing in-situ imaging and sensing analyses. • Time-gated analyses improve image and spectra by signal accumulation and noise reduction.
Source: Materials Today Bio
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,150
Award: G6843149321
OpenAlex ID: W4402536192