Work

Generation of RAB4A homozygous knockout induced pluripotent stem cell (iPSC) line

Year: 2024

Type: article

Abstract: The RAB4A gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in sorting and recycling. In this study, we generated induced pluripotent stem cells (iPSC) from a healthy individual by electroporation of peripheral blood mononuclear cells. We generated a RAB4A homozygous knockout human iPSC line via CRISPR/Cas9 gene editing. The iPSCs-RAB4A −/− had a normal karyotype, expressed pluripotency markers, and maintained trilineage differentiation potential.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2024.103556

Authors: Rui wang, Qing shen

Institutions: Chinese Academy of Sciences, Guangzhou Institutes of Biomedicine and Health, Guangzhou Medical University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,320