Assessment of safety: In vitro reverse mutation and in vivo acute oral toxicity tests of three biomass products from amino acid-producing Corynebacterium glutamicum
Year: 2024
Type: article
Abstract: Microbial fermentation has emerged as a pivotal process for sustainable production of essential goods and chemicals. Corynebacterium glutamicum is a proficient platform organism that contributes significantly to amino acid production through microbial fermentation. Despite its recognized safety, challenges persist in efficiently biosynthesizing natural products compared with other organisms. This study evaluated the safety of biomass products from bioengineered C. glutamicum through two different toxicological studies: a bacterial reverse mutation test (AMES test) and an acute oral toxicity test in rats. Three types of dried fermentation biomass products, each engineered for the enhanced production of specific amino acids (L-lysine, L-threonine, and L-tryptophan), were examined. The tests were conducted in compliance with Organization for Economic Co-operation and Development guidelines and revealed no mutagenicity or acute toxicity at the tested doses. These findings suggest the safety of biomass products from bioengineered C. glutamicum as potential feed materials, although further toxicity studies are recommended for comprehensive evaluation. This study underscores the importance of stringent safety assessments for advancing biotechnological applications and provides valuable insights into the potential utilization of microbial fermentation products in various industries. Moreover, this study highlights the significance of regulatory compliance and adherence to international standards to ensure the safety and efficacy of novel biotechnological products. • Microbial fermentation is essential for sustainable microbial production and Corynebacterium glutamicum a proficient platform organism in the industry. • The safety of biomass products from bioengineered C. glutamicum , which is used to produce the amino acids: L-lysine, L-threonine, and L-tryptophan, is evaluated in this study. • A bacterial reverse mutation test (AMES test) revealed that the biomass products are not mutagenic, thus not induce DNA mutation in cells. • An acute oral toxicity study in rats revealed that the biomass products do not show any adverse effect. • Therefore, three biomass products in this study are safe enough to be used as feed additives itself.
Source: Toxicology Reports
Authors: Ji-Eun Park, Jiyeon Kim, MiNa Baek, Hyo-Jin An, Chan-Sung Park, Joon Young Jung, So-Young Kim, Yang Hee Kim
Institution: CJ CheilJedang (South Korea)
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,230
OpenAlex ID: W4402564197