Work

Proceeding Abstracts (Invitation Only)

Year: 2024

Type: article

Abstract: LHMM24001 EFFECT OF GIGANTOL ON THE TNF-Α/NF-ΚB PATHWAY IN DIABETIC RETINOPATHY Yahan Luoa,b, Guifu Lib, Chunxia Lib aShanghai University of Traditional Chinese Medicine, Shanghai 201203, China,bDepartment of Ophthalmology, Shanghai TCM-Integrated Hospital, Shanghai University of TCM, Shanghai, 200082, China. Background: The purpose of this study was to investigate the effect of gigantol on retinal cell injury induced by high glucose (HG) culture in rhesus monkey choroidal-retinal endothelial (RF/6A) cells and in mouse models of diabetes in vitro and in vivo. Subjects and Methods: We firstly detected the effect of gigantol on proliferation of RF/6A cells with the CCK-8 kit. Secondly, we used an NF-κB reporter assay to determine whether gigantol has anti-inflammatory activity against the NF-κB gene. Thirdly, RF/6A cells were treated with different concentrations of gigantol (5, 10, or 25 μmol/ L) and cultured under HG conditions (30 mmol/ L) or TNF-α (5 ng/ ml) for 48 h. Afterwards, we observed changes in the TNF-α/NF-κB pathway protein in RF/6A cells, and detected the effect of gigantol on TNF-α/NF-κB pathway related gene expression in RF/6A cells. In a mouse model of STZ-induced (55 mg/kg) diabetes, the protein levels of TNF-α and interleukin-6 (IL-6) in the serum were analyzed using ELISA. The expression of NF-κB in the retinal tissue of each group was detected by immunohistochemistry and the protein and related gene expression of TNF-α/NF-κB pathway observed in mouse retina. Results: Our findings show that gigantol can inhibit NF-κB transactivity. Gigantol inhibited high glucose and TNF-α induced NF-κB expression in RF/6A cells. The mRNA and protein levels of inflammatory cytokines downstream of NF-κB were also reduced. In a mouse model of STZ-induced diabetes, gigantol reduced serum TNF-α and IL-6 expression levels and prevented inflammatory damage to the retina. Conclusions: Our data suggest that gigantol may exert anti-inflammatory effects by inhibiting the NF-κB signalling pathway in diabetic retinopathy. Our findings are valuable for understanding the mechanism of action of gigantol and may be useful for drug development or food supplement research for diabetic retinopathy. Acknowledgements This research was supported by grants from the Guoyi-qiangyou Foundation of Shanghai Hongkou (2022-2024) (HKGYQYXM-2022-13). LHMM24002 OBSERVATION OF THE THERAPEUTIC EFFECT OF POSTERIOR SCLERAL REINFORCEMENT IN THE TREATMENT OF PATHOLOGICAL MYOPIA IN DIFFERENT AGE GROUPS Enguang Dinga, Enliang Dinga, Dan Menga, Yongxia Zhanga, Hongyu Sua, Yongli Lia, Yuxuan Haob, Lijuan Suna aHanDan AiYan Ophthalmology Hospital, Handan Hebei 056008, China,bClinical Medicine, Hebei Medical University, Shijiazhuang, Hebei 050000, China. Background: To compare the efficacy and safety of posterior scleral reinforcement (PSR) in the treatment of pathological myopia in different age groups. Subjects and Methods: 27 patients (31 eyes) with pathological myopia who underwent PSR treatment were selected and divided into two groups based on age. Patients aged>18 years old were classified as Group A, and those aged ≤ 18 years old were classified as Group B. The follow-up period shall not be less than 6 months, and the best corrected visual acuity (BCVA), refractive index, axial length, and complications of the two groups of patients shall be observed before surgery, 1 day after surgery, 3 meters after surgery, and the last follow-up. Results: The follow-up time for Group A was (8.76±3.11) months, and for Group B was (9.00±3.08) months. There was no significant difference in BCVA between the two groups of patients at postoperative 1 day, 3 meters, and the last follow-up (P>0.05), and there was no difference in BCVA changes between the two groups during each follow-up period; The refractive index of the two groups of patients decreased compared to preoperative one day and three months after surgery (P<0.05). There was no significant difference in refractive index between the last follow-up and preoperative, and there was no difference in refractive index changes between the two groups of patients during each follow-up period; The axial length of the eyes of the two groups of patients was shortened compared to before surgery at 1 day, 3 months, and the last follow-up, and the difference was statistically significant. One day after surgery, three months after surgery, and the last follow-up, Group A had more axial reduction than Group B, and the difference was statistically significant. On the first day after surgery, 7 patients (7 eyes) in Group A experienced visual distortion. After 3 months of follow-up, all 7 patients had relieved visual distortion. Group B patients maintained stable retinal membrane structure during the follow-up period. Conclusions: PSR is a safe and effective method for pathological myopia. In short-term follow-up, the effect of shortening the axial length is better in adults than in children. LHMM24003 DIAGNOSTIC VALUE OF OAS3 IN CHILDHOOD-ONESET SYSTEMIC LUPUS ERYTHEMATOSUS Yangfan Huanga,b,c, Qinchun Pand, Zhijian Zhongb,c, Junming Wua,b,c aDepartment of Basic Science, YuanDong International Academy of Life Sciences, Hong Kong 999077, China,bExperimental Center of BIOQGene, YuanDong International Academy of Life Sciences, Hong Kong 999077, China,cSystems Biology Research Center, Biology Institute, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China,dSchool of Medicine & Health, Guangxi Vocational & Technical Institute of Industry. No.37 Xiuling Road, Nanning, Guangxi Zhuang Autonomous Region, China. YH and QP contributed equally to this work. Background: Childhood onset systemic lupus erythematosus (cSLE) is a complex multisystem autoimmune disease, characterized by immune dysregulation and systemic inflammation, leading to progressive and irreversible multi organ damage. The objective of this study is to identify diagnostic markers for cSLE based on bioinformatics analysis and machine learning algorithms. Subjects and Methods: Gene expression profiles for cSLE were obtained from GPL96 and GPL97 platforms of GSE8650, and GSE11909 datasets then merged de batches. Differentially expressed genes (DEGs) were identified in both platforms and common DEGs with consistent direction of expression were identified through intersection analysis. Then we performed enrichment analysis for common DEGs. Common DEGs with area under receiver operating characteristic curve (AUC) values > 0.8 in both GPL96 and GPL97 platforms were used to build risk model with XGBoost algorithm model. The most important variable in model was further identified as key gene. Possible immune regulatory mechanisms of key gene were explored by identifying association with immune cells, which analyzed by single-sample gene set enrichment analysis, and cell death that differed between cSLE and control were also analyzed. Results: A total of 4219 DEGs in the GPL96 platform, and 4860 DEGs in the GPL97 platform, with 314 DEGs were upregulated expression with the same direction in GPL96 and GPL97, and 314 DEGs were downregulated expression with the same direction. Then 628 common DEGs were identified, they enriched in immune response, neutrophil extracellular trap formation, and NOD−like receptor signaling pathway. There were 26 common DEGs with AUC values > 0.8 in both GPL96 and GPL97 platform and then a XGBoost model was constructed. Subsequently, OAS3 was identified as a key gene with the biggest AUC value and important ranking. In addition, activated DCs were higher in cSLE and positively correlated with OAS3. Apoptosis, autophagy, necroptosis, and pyroptosis were significantly activated in cSLE and positively correlated with OAS3. Conclusion: This study constructed a diagnostic model and identified OAS3 as a key diagnostic marker. OAS3 may regulate immune responses and NOD−like receptor signaling pathway involved in cSLE. OAS3 may as a diagnostic for cSLE and may as a for A ON IN THE OF aDepartment of University, of of Hospital, and Research Medical and Research Medical China. Background: This study to investigate the effects on by with the Chinese Subjects and Methods: the and the a was at a with on the and and and in this were were divided into an group and a control with in The control group the group with the Chinese on of the The for 7 with a each and after the were using the Chinese and and the Medical with using Results: to the there were no statistically significant between the control group and the group in of > and > the the group on compared to the control group in the the was higher in the group compared to the control group the to and were in the group compared to the control group with statistically significant Conclusions: The of with Chinese and the a significantly effect on ON OF IN Lib of of and China. Background: the of of and the proliferation of in the for effective is more than under conditions of Traditional are for This study to this by two to in Subjects and Methods: This two a method based on and a method the and of were compared against and on characterized by Results: that both and in of and efficacy under The method effective for the method in and for in Conclusions: The findings that the for and for in a and for in the for as more and the and of this research significantly to the safety and of data in This was supported by Research and Research and and Research of A University, of China. Background: is a leading The of based on to the in compared to Subjects and Methods: We to a model for based on The model three models constructed in with The were in model to the for We and the model for with the culture of as the A with of was used for and the model by The was used for model We model by the to the of the model was analyzed for by as a Results: The model a high in The diagnostic of the model was higher compared to the on the of as as the of the model a on the that the and was higher than The of the model that the model the compared to the Conclusions: Our model a high in based on of the of the of the model and in This study was by and of OF ON THE IN of and University, China. Background: The of is related to the changes in and they the same as the changes in the of In the of and important that the of of In addition, of the of of which and After the the and are the three most of the after the The and of three are also the three for the and of the and This the data of in the in to study the effect of of on of after the changes in and changes and the different and then the on the of after Results: with who are with the of the of who are with the of by and with who can the of those who the of by with with with a in Conclusion: The of with the of for and the to a significant on the of This study was supported by the IN IN Lia, of Science, University, of Center of of and China. Background: The of with the of significant for in of and in the of and in The of this data to This study to the and of analysis and by a visual analysis to Subjects and Methods: To the by this the and of a visual analysis for based on visual analysis The was through on in and The to the to data and the and analysis of Results: The study that the visual analysis identified in time The significantly reduced the on by and This in data more and of the to in Conclusions: This research that the visual analysis for significantly the and of in and and data the better positively to the and of This is for the and of data in in the analysis and of and in This was supported by Research and Research and of of and Research of PATHWAY IN OF Hong Zhanga, aDepartment of of Autonomous Region, China,bDepartment of Medical University of the China. Background: the and analysis of pathway based with in of Subjects and Methods: patients with in the of and of a in from to were selected research to the of patients from to patients were used the control and patients from to were used the The control group and follow-up, patients were and On the of the control the group pathway with a pathway a with and follow-up months after visual activity index index and of were used to the effect of Results: the the of and in the two groups were not statistically significant months after the the of and in the group were significantly than those in the control and the of were significantly higher than those in the control group (P<0.05). Conclusions: The pathway with the of and has effects on has in and of has high value in and is and This research was supported by Autonomous Research and Research and and Autonomous and OF IN THE OF Lia, of and University, China. This study to the of in the and of in the the of in Chinese and the of and we are to using to and of the safety and of Chinese and in the Methods: This study the research on in the of in and on the and of in the and of on different and of the were and the of was by analysis and Results: The research that can the characteristic of in the of the of from different and was that there is a between the in and the In addition, can also be used for the analysis of and efficacy of for control and of Chinese Conclusions: has in the and of in the This not has the of and also of Chinese and there are and in the for the of as the of of of of and In the with the of and the of a more important in the more and effective for the safety and control of Chinese Acknowledgements This was supported by University Basic Research and University Basic Research THE OF IN OF Lia, Hospital, of Medicine, University, China,bDepartment of Hospital, China. Background: can the of cells is the of by cell during This study the mechanism of in the and of Subjects and Methods: The expression of in of patients and and and in were and related were and expression in The signaling pathway were to whether the expression of the during the of to Results: The expression of changes significantly during higher levels in patients compared to that of in significantly the expression of which the signaling pathway and the signaling significantly the markers with as activity and The of also in higher expression levels of which to with to analysis that of the of There is a in by and with a reduction in as This is further supported by the of the signaling pathway and a of a reduction in Conclusions: and a in the of by the of into The study that through signaling an of and of and and ON Lib, aDepartment of to Medical University, China,bDepartment of to University, China. Background: or is a by and years with a through three inflammatory and The significantly of to and with and no and This study the efficacy and safety of with in patients using and IL-6 levels as To the efficacy of with for Methods: patients with to the of Medical University from to were divided into two groups to the The control group with and the study group with The and serum interleukin-6 (IL-6) were compared before and 1 and 3 months after were also Results: After the at 1 and 3 months decreased in both groups compared to those before with statistically significant there was no statistically significant difference between the two groups After the at 1 and 3 months in both groups compared to those before with statistically significant and the study group was better than the control with a statistically significant difference (P<0.05). The serum IL-6 at 1 after treatment decreased compared to that before with a statistically significant difference there was no statistically significant difference between the two groups complications in the of and at the was in the study group than in the control group (P<0.05). Conclusion: with and with can in patients with the has better and This was supported by a from for Medical University OF A OF of and University, of and Science, Academy of & of Institute of and of University of Background: the last years Chinese has as a Chinese is related to the of which has based on different of or short-term with a stable and to a can Subjects and Methods: The in this is to the and of in the of and This is an of Chinese that is to the of of The is divided into The first the of and The the of in the of Chinese the of the of from to and and The of the of and on Chinese and The the for the and of in and are a stable and can a of and in a more to the development of and can and between more than which is to and Results: The from to of is to the of Chinese was in a or of and during the period in the of this was in a more or by after was in the the of a on Chinese and the of Chinese has in of the significant on and The of also of and which can effects on as of and Conclusions: is to for and the from to short-term of is to of Chinese the of a on Chinese and with a stable and to a can The that or an in into OBSERVATION ON THE TREATMENT OF of University, Hospital, Hospital, Center, China. Background: This study observed the efficacy of with in the treatment of and a Subjects and Methods: a patients with were selected as the study and were divided into a treatment group and a control group in the of with in each The as and age between the two groups is the the control group maintained the of of the through After the the of treatment to and using The treatment group was treated with with the treatment the selected was at of the of the and on the of the The used to and the into the selected and to the before the the was to with To the was used to changes in the the same were used to the of the and the in and Results: After analysis, there was no significant difference in the values between the two groups of patients before treatment After of the effective of both groups were significantly (P<0.05). the effective of the treatment group was significantly higher than the control and the difference was statistically significant (P<0.05). The of both groups were significantly than before treatment and the of the treatment group were than those of the control with (P<0.05). The of the and groups in both groups of patients was significantly by a significant in values compared to before to the control the values of the and in the treatment group were and this difference was statistically significant (P<0.05). Conclusions: under the of with the of the effect of and and the effect of The two each each and can at the same This method was for patients to in the to the significantly of and the of This was supported by a from The Traditional Chinese Medicine aDepartment of Shijiazhuang, Hebei China. Background: To investigate the safety and efficacy of in patients with treated with Subjects and Patients with treated with were and divided into three groups to the treatment of and data were analyzed the and and Results: A total of patients were patients in the group and in the Patients in the group were further divided into the and significant in the data or the between the three groups. was in all patients and the was 3 in all patients after significant were in the and between the and the groups at follow-up. follow-up, in or patients in the in or in the and in or in the One in the group also experienced the which was to be related to the to as by the patients experienced or in the or or in the with the group or the there was no significant difference in and in the Conclusion: The of is as safe and effective as and in and at and follow-up in patients with treated with which may be used to treatment in patients with This study was supported by the of and of University, of University of & University, The Background: is a that in in the and which was by and groups with different The years and most of In to and is the of in the was also to a and in in is one of the of the and a leading in the in into that the machine also has a and the and in the and a there are and that from a is a by or Patients to a of and they may be in they may also or of this with of and Subjects and Methods: This to an analysis of in and as and and and important in and the of on from the of Results: the analysis of and and is that is not the of patients with also has a of effect on on and also which to the of and the of and time and Conclusions: Our and are to and and the of has important value for with and the to the In the the most may a and a of to the effect of on and we to as a to to the treatment of patients with the same through the research of this we on the to more to with to in and and to to in a they are and and into they may be the Acknowledgements This was supported by a from and to Research on and of IN THE aDepartment of University of China,bDepartment of University of China. Background: The research on on as and on the different of In the of are the as and the they and is also an important Subjects and Methods: This from a in the and the in the of This the method of and to the of the as to an in the of and and compare the of in the of in the the between and Results: In the has to the of and in an and in the is to show in is by is the of for no or The of in in and also the of in This the between and an that be Conclusions: the of for both and as as the in in and to the research on between and This a for and the that are by and by from the of can in a for the risk of research into and also to in This was supported by and from the Research for the and on the in from Research Center OF Lib, Institute of and of and University, Life and Group China. To research of and activity using Subjects and Methods: are a of in and to as and the risk of disease, which the activity has In this were and were classified into the activity of was using and were by analysis to the between the and the activity of Results: This study selected to the different and of and activity and using the A of of was Conclusions: The of each to activity in different was also The had the on the activity in and the activity in and was by the of and the of Acknowledgements The to Institute of and the Foundation of the Research of of and University and the Basic and Basic Research Foundation OF IN Zhanga, and China. This study to the of models in with a on data analysis, and the research to the of and analysis, and and Subjects and Methods: The study a and analysis to the of in were used to the of in data analysis, and in Results: The findings that a of in the of we can the and of as as the and of can in better understanding and more In the of is to changes to the the of and Conclusions: The of in is both and research is to the of in to in This study and for the development of the a more and OF IN OF THE OF aDepartment of China,bDepartment of and University of and of and University, of University of and China. Background: for a of and In has in the and development of characteristic to A of research has at the and development of the of a in the development of research on in on and with to the of This study analysis to the research and in of China. for development of from the of to identify for development that to both and of and of Subjects and Methods: This research on the in from the as the research This study the analysis as a to a analysis of the research of in This study to a and of research on which the research and direction for the development of Results: The into in in a in and a is with a of higher The research is The study has and the for the the and the of Conclusions: the study of in of has with the of research and research more is The study through and development to and research more and OF of Vocational and Technical University of China,bDepartment of Science, Vocational and Technical University of China. Background: The objective of this is to a of the for to the of in and for in this Subjects and Methods: The research in this study key the an of the research and by a The and related to and a for the Secondly, the to this as and are to a The of the of and The of on the of and to Subsequently, the of for and the of are analyzed. This analysis the of identifying the and that a is to the of for The data from a of to and of The obtained data is then analyzed to identify the and a for is based on the research This for and in Results: on the analysis of the for are and to to and a of The

Source: Medicine

DOI: 10.1097/md.0000000000038805

Language: English

Topic: Retinal Diseases and Treatments

Subfield: Ophthalmology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,950