Work

Development of a programmable automated cell culture system to study the lung pathophysiology of Cystic Fibrosis-related diabetes

Year: 2024

Type: article

Abstract: are initially exposed to high glucose media. Glucose gets progressively depleted until the next media change days later, which is not physiologically relevant and could negatively impact the results of research studies. To better study the mechanisms driving CFRD pathophysiology, we developed a programmable and automated cell culture system (PACCS) capable of mimicking acute hyperglycemic episodes experienced by CFRD patients after meals. We adapted a commercially available perfusion system and performed 3D modeling to develop this system. Results show that PACCS can be successfully used to culture airway epithelial cells, both immortalized and primary cells. Further, CF cells responded differently to meal-like conditioning when compared to controls, suggesting impaired adaptative responses in CF cells. Overall, PACCS will allow us to better study CFRD pathophysiology, and it could be used for a wide range of other applications.

Source: Heliyon

DOI: 10.1016/j.heliyon.2024.e37977

Authors: Analia J. Vazquez Cegla, Cameron Hedden, Barry R. Imhoff, Guiying Cui, Nael A. McCarty

Institutions: Emory University, Children's Healthcare of Atlanta

Language: English

Topic: Cystic Fibrosis Research Advances

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,100