Type Three Secretion System-induced Mechanoporation of Vacuolar Membranes
Year: 2024
Type: preprint
Abstract: S ummary Endomembrane breaching is a crucial strategy employed by intracellular pathogens enclosed within vacuoles to access the nutrient-rich cytosol for intracellular replication. While bacteria use various mechanisms to compromise host membranes, the specific processes and factors involved are often unknown. Shigella flexneri, a major human pathogen, accesses the cytosol relying on the Type Three Secretion System (T3SS) and secreted effectors. Using in-cell correlative light and electron microscopy, we tracked the sequential steps of Shigella host cell entry. Moreover, we captured the T3SS, which projects a needle from the bacterial surface, in the process of puncturing holes in the vacuolar membrane. This initial puncture ensures disruption of the vacuolar membrane. Together this introduces the concept of mechanoporation via a bacterial secretion system as a crucial process for bacterial pathogen-induced membrane damage. G raphical A bstract
Institutions: Centre National de la Recherche Scientifique, Institut Pasteur, Université Paris Cité, Biologie cellulaire physiologique et pathologique, Institut de Chimie des Substances Naturelles, Department of Virology, Harvard University, Massachusetts General Hospital, Inserm, Centre Hospitalier Universitaire de Nantes, UMS BioCore, Structure Fédérative de Recherche Bonamy, Nantes Université
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4402625462