Ionic release from bioactive SiO2-CaOCME/poly(tetrahydrofuran)/poly(caprolactone) hybrids drives human-bone marrow stromal cell osteogenic differentiation
Year: 2024
Type: article
Abstract: This study demonstrates that dissolution products of inorganic/organic SiO 2 -CaO CME /PTHF/PCL-diCOOH hybrid (70S30C CME -CL) drive human bone marrow stromal cells (h-BMSCs) down an osteogenic pathway with the production of mineralised matrix. We investigated osteogenesis through combined analyses of mRNA dynamics for key markers and targeted staining of mineralised matrix. We demonstrate that h-BMSCs undergo accelerated differentiation in vitro in response to the 70S30C CME -CL ionic milieu, as compared to incubation with osteogenic media. Extracts from 70S30C CME -CL promote osteogenesis by inducing changes in cellular metabolic activity, promoting changes in cell morphology consistent with the osteogenic lineage, and by enhancing mineralisation of hydroxyapatite in the extracellular matrix. Additionally, our results show that 70S30C CME -CL hybrids prove sustained functional resilience by maintaining osteostimulatory effects despite cumulated dissolution cycles. In co-differentiation medium, 70S30C CME -CL ionic release can modulate signalling pathways associated with non-osteogenic functions, further supporting their potential for bone regeneration applications. Overall, our study provides compelling experimental evidence that the 70S30C CME -CL hybrid is a promising biomaterial for bone tissue regeneration applications. • 70S30C CME -CL hybrid material is biocompatible. • 70S30C CME -CL mediate metabolic and morphological processes towards osteogenesis. • 70S30C CME -CL ionic release enables mineralised extra-cellular matrix production. • 70S30C CME -CL ionic release enhances key osteogenic markers expression. • Maintained osteostimulatory effects of 70S30C CME -CL hybrid over dissolution cycles
Source: Biomaterials Advances
Institution: Imperial College London
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,910
Awards: Biodegradable hybrid screws for ligament-bone interface regeneration, A Superresolution microscope for biological research in the multi-user Microscopy Facility [FILM]: ALERT 13, 3D printing multifunctional devices without internal interfaces for cartilage repair, G2948680474, G370930089, G3756107532, G4736358383, G55772435, G6136284459, G6501379603, G869244616
OpenAlex ID: W4402629650