Year: 2024
Type: article
Study design: Observational Study
Abstract: Aim: Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.Materials & methods: Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.Results: Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.Conclusion: This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.
Source: Epigenomics
Authors: Paulo Victor Barbosa Eleutério dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Award: G7146146314
OpenAlex ID: W4402634089