Post-surgery sequelae unrelated to disease progression and chemotherapy revealed in follow-up of patients with stage III colon cancer
Year: 2024
Type: article
Study design: Observational Study
Abstract: BACKGROUND: We studied the poorly-known dynamics of circulating DNA (cir-nDNA), as monitored prospectively over an extended post-surgery period, in patients with cancer. METHODS: On patients with stage III colon cancer (N = 120), using personalised molecular tags we carried out the prospective, multicenter, blinded cohort study of the post-surgery serial analysis of cir-nDNA concentration. 74 patients were included and 357 plasma samples tested. FINDINGS: ), in accordance with previous reports which indicate that cir-nDNA are NETs by-products. Unexpectedly, in 34 out of 50 patients we found that NETs continued to be formed for an extended duration post-surgery, even in patients without disease progression. Given that this phenomenon was observed in patients without adjuvant CT, and in patients >18 months post-surgery, the data suggest that the persistence of NETs formation is not due to the adjuvant CT. INTERPRETATION: (1), Given the inter-patient heterogeneity, the post-surgery cir-nDNA level cannot be considered a reliable value, and caution must be exercised when determining mutation allele frequency or the mutation status; and (2), specific studies must be undertaken to investigate the possible clinical impact of the persistent, low-grade inflammation resulting from elevated NETs levels, such as observed in these post-surgery patients, given that such levels are known to potentially induce adverse cardiovascular or thrombotic events. FUNDING: This work was supported by the H2020 European ERA-NET grant on Translational Cancer Research (TRANSCAN-2).
Source: EBioMedicine
Authors: Alexia Mirandola, Andrei Kudriavtsev, Catalina Isabel Cofre Muñoz, Raquel Comas Navarro, Marco Macagno, Saidi Daoud, Cynthia Sanchez, Brice Pastor, Ekaterina Pisareva, Mireia Sanchis Marin, Javier Gonzalo Ruiz, Alejandro Piris, Ariadna Garcia Rodriguez, Nadia Saoudi Gonzalez, Ana Vivancos, Virginia Quarà, Alfredo Mellano, Felice Borghi, Giorgio Corti, Caterina Marchiò, Anna Sapino, Alice Bartolini, Giovanni Crisafulli, Alberto Bardelli, Massimo Di Maio, Gerald Lossaint, Florence Frayssinoux, Evelyne Crapez, Marc Ychou, Ramon Salazar Soler, Elisabetta Fenocchio, Paula X. Fernandez Calotti, Thibault Mazard, Cristina Santos Vivas, Elena Elez, Federica Di Nicolantonio, Alain R. Thierry
Institutions: Inserm, Université de Montpellier, Institut de Recherche en Cancérologie de Montpellier, Institut d'Investigació Biomédica de Bellvitge, Institut Català d'Oncologia, Vall d'Hebron Institute of Oncology, Candiolo Cancer Institute, University of Turin, IFOM, Institut Regional du Cancer de Montpellier, Universitat de Barcelona
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,000
Funders: Fundación Cellex, Institut National de la Santé et de la Recherche Médicale, Fundación Científica Asociación Española Contra el Cáncer, Generalitat de Catalunya, Ministero della Salute, Institut National Du Cancer, Direction Générale de l’offre de Soins, Fundación Fero, Horizon 2020 Framework Programme, Agència de Gestió d'Ajuts Universitaris i de Recerca, Instituto de Salud Carlos III
Awards: G1722846474, G607287237, G8824911526
OpenAlex ID: W4402635217