Work

Impairment of oocyte quality caused by gut microbiota dysbiosis in obesity

Year: 2024

Type: article

Abstract: Obesity poses risks to oocyte maturation and embryonic development in mice and humans, linked to gut microbiota dysbiosis and altered host metabolomes. However, it is unclear whether symbiotic gut microbes have a pivotal role in oocyte quality. In mouse models of fecal microbiota transplantation, we demonstrated aberrant meiotic apparatus and impaired maternal mRNA in oocytes, which is coincident with the poor developmental competence of embryos. Using metabolomics profiling, we discovered that the cytosine and cytidine metabolism was disturbed, which could account for the fertility defects observed in the high-fat diet (HFD) recipient mice. Additionally, cytosine and cytidine are closely related with gut microbiota dysbiosis, which is accompanied by a notable reduction of abundance of Christensenellaceae R-7 group in the HFD mice. In summary, our findings provided evidence that modifying the gut microbiota may be of value in the treatment of infertile female individuals with obesity. • HFD and ob/ob mice have similar bacterial composition, which are different to ND. • Oocyte quality and embryonic development are impaired in HFD and OB recipient mice. • Pyrimidine metabolism is disrupted in HFD recipient mice. • Cytosine and cytidine influence oocyte quality in a gut microbiota-dependent manner. • Cytosine and cytidine are closely related with Christensenellaceae R-7 group.

Source: Genomics

DOI: 10.1016/j.ygeno.2024.110941