Work

Identification of daurisoline metabolites in rats via the UHPLC-Q-exactive orbitrap mass spectrometer

Year: 2024

Type: article

Abstract: Daurisoline, a bisbenzylisoquinoline alkaloid extracted from the rhizomes of Batrachuperus, exhibits diverse biological activities, encompassing antiplatelet, anti-inflammatory, neuroprotective, and antitumor properties. However, previous investigations have not comprehensively elucidated the metabolic profile and pathways of daurisoline in vivo. Using UHPLC-Q-Exactive Orbitrap MS technology, we comprehensively investigated the metabolites of daurisoline in Sprague-Dawley rats, following oral administration. Data collection and analysis were enhanced through Full Scan MS/dd-MS2, in conjunction with parallel reaction monitoring, extracted ion chromatography, and diagnostic fragment ions. Sixty-three metabolites were detected and characterized, including sixty-two novel metabolites and coclaurine. This investigation elucidated the cleavage patterns and tissue dissemination inherent to daurisoline metabolism. Furthermore, in vivo reactions, including dehydrogenation, hydroxylation, methylation, sulfation, glucuronidation, and intricate combinations thereof, were thoroughly examined. Investigating the metabolites of daurisoline in rats has deepened our understanding of its metabolism in vivo, aiding in elucidating its metabolic and pharmacological actions. This provides a valuable foundation for further research into its therapeutic efficacy.

Source: Journal of Pharmaceutical and Biomedical Analysis

DOI: 10.1016/j.jpba.2024.116482

Authors: Ruijun Cai, Jing Liu, Xuefang Wang, Tao An, Ling Zhang

Language: English

Topic: Traditional Chinese Medicine Analysis

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,540

Funder: Jinan Science and Technology Bureau