Work

Andrology laboratory review: evaluation of sperm morphology

Year: 2024

Type: article

Abstract: Sperm morphology assessment has an important role in male fertility diagnosis and prognosis, both for humans and animals. Thus, it is important that relevant results are comparable and consistent. To achieve these aims, the following procedures are recommended: a. semen sample is suitably ‘fixed’ (e.g. in isotonic buffered formal-saline); b. sperm are examined at 1,000 x (phase or DIC microscopy); c.at least 200 sperm are counted; d. each sperm is placed into 1 category, only (e.g. normal, head, midpiece etc), and e. 70% ‘normal’ sperm is the threshold for a satisfactory sample. In addition, morphologists should be provided with relevant continuing education, upskilling, and monitoring programs. This review provides guidelines for the best performance of this assessment, as well as for avoiding pitfalls.

Source: Clinical Theriogenology

DOI: 10.58292/ct.v16.10600

Authors: Peter Chenoweth, Leo Brito, Augustine Peter, Dagmar Waberski, Gary Althouse, Christine Aurich, Gaia Luvoni, Regina Turner, Natalie Fraser, Cheryl Lopate

Institutions: James Cook University, University of Pennsylvania, Purdue University West Lafayette, University of Veterinary Medicine Hannover, Foundation, University of Veterinary Medicine Vienna, University of Milan, The University of Queensland

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4402643576

FWCI: 1.638

Cites:

Cited by: 5

Related to: 10