Work

A Surface-Mediated Biomimetic Porous Polyether–Ether–Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis

Year: 2024

Type: article

Abstract: The repair of large bone defects remains a major challenge for clinical orthopedic surgery. Here, we develop a surface biofunctionalized three-dimensional (3D) porous polyether-ether-ketone (PEEK) scaffold that can simultaneously promote osteogenesis and regulate macrophage polarization. The scaffold is designed by polydopamine (PDA)-assisted immobilization of silk fibroin (SF) and electrostatic self-assembly of nanocrystalline hydroxyapatite (nano-HA) on a 3D-printed porous PEEK scaffold. The SF/nano-HA functionalized surface provides a bone-like micro-environment for osteoblastic cells adhesion, proliferation, mineralization and osteogenic differentiation. Moreover, the biofunctionalized surface can effectively drive macrophages polarization from pro-inflammatory M1 phenotype to anti-inflammatory M2 phenotype. Integrin β1 specific cell-matrix binding and the activation of Ca2+ receptor-mediated signaling pathway play critical roles in the regulation of macrophage polarization. Compared with the as-printed one, the SF/nano-HA functionalized porous PEEK scaffold induces minimal inflammatory response, enhanced angiogenesis and substantial new bone formation, and thus results in improved osseointegration in vivo. This study not only develops a promising candidate for bone repair but also demonstrates a facile surface biofunctionalization strategy for orthopedic implants to improve osseointegration by stimulating osteogenesis and regulating immunity.

Source: ACS Biomaterials Science & Engineering

DOI: 10.1021/acsbiomaterials.4c00725