Work

Bioactive peptides from food waste: New innovative bio-nanocomplexes to enhance cellular uptake and biological effects

Year: 2024

Type: article

Abstract: Mastitis is the most important bovine disease, causing dramatic economic losses to the dairy industry, worldwide. This study explores the valorization of whey from cows affected by mastitis, through a novel separation approach. Surface Active Maghemite Nanoparticles (SAMNs) were used as magnetic baits to selectively bind bioactive peptides with potential health benefits. Advanced techniques such as HPLC and LC-MS/MS highlighted SAMN capability of isolating a restricted group of peptides, drastically diverging from the control profile (Solid Phase Extraction, SPE) and characterized by a peculiar acidic residue distribution. Most importantly, both magnetically purified and nano-immobilized peptides (SAMN@peptides) showed protective activity against oxidative stress and inflammation, when tested on Caco-2 cells; with SAMN@peptides being associated with the strongest biological effect. SAMNs exhibited excellent characteristics, they are environmentally sustainable, and their synthesis is cost-effective prompting at a scalable and selective tool for capturing bioactive peptides, with potential applications in functional foods and nutraceuticals. • Milk whey from mastitis affected cow valorization by extracting bioactive peptides. • Functional bio-corona on peptide selective nanoparticles was explored. • New bioactive peptides protect human cells against oxidative stress and inflammation. • Nanoparticle-peptide hybrids show a high biological activity on human cells. • The proposed nanotechnological strategy can be used in food and health fields.

Source: Food Chemistry

DOI: 10.1016/j.foodchem.2024.141326