Work

Effects Of Short-term Tai Chi On Circulating Oxylipins And Endocannabinoids In Post-menopausal Women

Year: 2024

Type: article

Abstract: BACKGROUND/OBJECTIVES: We recently reported differences in circulating levels of pro-inflammatory oxylipins (OxL) and endocannabinoids (eCB) among postmenopausal women with knee osteoarthritis after 8 weeks of Tai Chi (TC) (Shen et al. 2023, doi: 10.3389/fmed.2023.1210170). This study examined if plasma eCB/OxL levels change after 4 sessions of TC within a 10-day period in postmenopausal women. METHODS: Sixteen post-menopausal women completed 4 TC sessions on non-consecutive days within 10 days, 60 min/session including 5 min of warmup, 50 min practicing TC, and 5 min of cooldown. Participants learned 10 selected moves from 24-form Yang-style TC in sections designed to allow self-practicing in a short time. Plasma isolated from non-fasting blood draws at baseline and immediately after TC session 4 were analyzed for eCB (e.g., AEA and 2-AG) and OxL using targeted metabolomic methods that preserve 2-AG (Pedersen et al. 2021, doi: 10.1016/j.aca.2020.11.019). Specifically, oxylipins were isolated by solid phase extraction on 10 mg Oasis-HLB sorbent beds (Waters Corp, Milford, MA) as previously described (Luria et al. 2007, doi: 10.1074/jbc.M608057200), modified for use in a 96 well plate format. Partial least squares discriminant analysis highlighted changes between pre- and post-intervention samples. Paired t-tests with false discovery rate corrections were performed to examine the differences in plasma OxL and eCB. RESULTS: 16 women (58.9 ± 5.2 years old, BMI: 33.6 ± 5.4 kg/m2) completed the study. Few fatty acid values differed from baseline after the 4th TC session. The plasma 20:5n3 metabolite 18 HEPE, 18:2n6, 18:3n3, the 18:3n3 OxL metabolite 15,16-DiHODE, and multiple 18:2n6-derived metabolites were increased, while monoacylglycerols (e.g., 1-AG) and the 12-lipoxygenase metabolites 12-HETE and 14-HDoHE were lower after TC. CONCLUSIONS: The short-term 4 TC sessions in post-menopausal women had relatively little impact on eCB and OxL in self-reported healthy post-menopausal women. The lower levels of monoacylglycerols and higher PUFA 18:2n6 suggest that TC might mobilize fatty acids and involve enzymes of 1-AG metabolism such as phospholipase C, DAGL-alpha, MGL, and perhaps FAAH. A limitation in this study is the non-fasted state of the subjects. NIH/NINDS R01 NS038261 (VN), Texas Tech University Neuroimaging Institute, Lubbock, TX (CLS) and the Center of Excellence for Translational Neuroscience and Therapeutics, Texas Tech University Health Sciences Center, Lubbock, TX (CLS/VN). USDA Intramural Projects 2032-51530-025-00D (JWN).

Source: Medicine & Science in Sports & Exercise

DOI: 10.1249/01.mss.0001059028.93651.68