Work

ER–plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion

Year: 2024

Type: article

Abstract: As a consequence of hypoosmotic shock, yeast cells swell rapidly and increase the surface area by ∼20% in 20 s. Approximately, 35% of this surface increase is mediated by the ER-plasma membrane contact sites, specifically the tricalbins, which are required for the delivery of both lipids and the GPI-anchored protein Crh2 from the cortical ER to the plasma membrane. Therefore, we propose a new function for the tricalbins: mediating the fusion of the ER to the plasma membrane at contact sites. This proposed fusion is triggered by calcium influx via the stretch-gated channel Cch1 and is supported by the anoctamin Ist2.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.202308137

Authors: Madison Smith, Lincoln Gay, Markus Babst

Institution: University of Utah

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G2135684186, G3937335605, G9000170644