Work

Cryogenic probe technology enables multidimensional solid-state NMR of the stratum corneum without isotope labeling

Year: 2024

Type: article

Abstract: Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum , the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides , and triacylglycerols , as well as enables 1 H– 1 H spin diffusion monitored by 2D 1 H- 13 C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids . The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.

Source: Solid State Nuclear Magnetic Resonance

DOI: 10.1016/j.ssnmr.2024.101972

Authors: Barbara Perrone, Maria Gunnarsson, Diana Bernin, Emma Sparr, Daniel Topgaard

Institutions: Bruker (Switzerland), Lund University, Chalmers University of Technology

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,040

Funder: Bo Rydins Stiftelse för Vetenskaplig Forskning