Work

Circulating exosomal protein EFEMP1 and SERPINC1 as diagnostic biomarkers for epithelial ovarian cancer

Year: 2024

Type: article

Abstract: • MS/MS analyses identified a total of 207 differential exosomal proteins, of which 122 were up-regulated and 85 were down-regulated. • The levels of exosomal EFEMP1 and SERPINC1 were significantly upregulated in EOC patients as compared to healthy controls and benign disease patients, processing favorable diagnostic efficiency for EOC. • Exosomal EFEMP1 and SERPINC1 exerted rather high early diagnostic efficiency, as well as the potential to distinguish the malignant patients from the benign individuals. Besides, exosomal SERPINC1 was associated with coagulation index and LE-DVT (lower extremity deep venous thrombosis) in EOC patients • Exosomal SERPINC1 was associated with LE-DVT (lower extremity deep venous thrombosis) in EOC patients and can be used as an independent predictor of LE-DVT. • Exosomal EFEMP1 and SERPINC1 can serve as potential biomarkers for EOC. Caner-derived exosomes, containing diverse nucleic acids and proteins, are being exploited in diagnostic biomarker development. This study aims to screen and identify the altered exosomal proteins between epithelial ovarian cancer (EOC) patient and healthy volunteers, and to evaluate their diagnostic accuracy for EOC. Exosomes were separate by ultracentrifugation, and then subjected to TEM, qNano, and western blot for identification. Exosomal EFEMP1 and SERPINC1 were selected by MS/MS analysis, validated by ELISA in a cohort with 163 healthy donors, 183 EOC patients and 30 patients with benign ovarian tumors. MS/MS analyses identified a total of 207 differential exosomal proteins, including the 122 up-regulated and 85 down-regulated. Exosomal EFEMP1 and SERPINC1 were significantly upregulated in EOC patients compared with those in healthy donors as well as in the benign patients, possessing favorable diagnostic efficiency. The area under the curves (AUCs) were 0.8071, 0.8211, respectively. They also exerted rather high early diagnostic efficiency, as well as the potential to distinguish the malignant patients from the benign individuals. Besides, exosomal SERPINC1 was associated with coagulation index and LE-DVT (lower extremity deep venous thrombosis) in EOC patients. Exosomal EFEMP1 and SERPINC1 are upregulated and serve as the promising diagnostic biomarkers for EOC.

Source: Translational Oncology

DOI: 10.1016/j.tranon.2024.102126

Authors: Shiwen Wang, Huimin Wang, Kangyu Wang, Qianru Zhang, Xingguo Song

Institutions: Harbin Medical University, Shandong Tumor Hospital, Shandong First Medical University

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,130