Incidence and Antibiotic Resistance Patterns of Staphylococcus aureus and Pseudomonas aeruginosa Isolated from Fomites in Critical Units of General Hospital Katsina
Year: 2024
Type: article
Abstract: Nosocomial infections, predominantly caused by Staphylococcus aureus and Pseudomonas aeruginosa, continue to be a major public health challenge, especially in developing countries, due to their associated morbidity and mortality. This study aimed to isolate and identify bacterial pathogens from fomites in the Accident and Emergency (A&E) unit, male ward, and female ward of General Hospital Katsina and to evaluate their antimicrobial susceptibility patterns. A total of 90 swab samples were collected from frequently touched items such as beddings, door handles, floor surfaces, scissors, and forceps across the three locations. Standard microbiological techniques were utilized for the isolation and identification of S. aureus and P. aeruginosa, followed by antibacterial susceptibility testing using the disc diffusion method. Bacterial growth was observed in 58 (64.4%) samples, with S. aureus accounting for 32 (55.2%) and P. aeruginosa for 26 (44.8%) of the isolates. The male ward exhibited the highest contamination levels, with a mean colony count of 725.25 CFU/plate, significantly higher than the female ward (487.67 CFU/plate) and the A&E unit (48 CFU/plate), as confirmed by a one-way ANOVA (p < 0.05). Chi-square analysis revealed no statistically significant association between bacterial species and hospital location (p = 0.823). The susceptibility testing showed that S. aureus isolates were generally susceptible to Gentamycin, Ciprofloxacin, and Pefloxacin but resistant to Amoxicillin. Conversely, P. aeruginosa displayed high resistance to Gentamycin and Septrin. Conclusively, these findings revealed the critical role of fomites in the transmission of pathogens and highlighted the need for enhanced infection control measures and targeted antibiotic stewardship programs to mitigate the risks associated with these infections.
Authors: Kaware, M. S., Abdul, N. A., Abdulkadir, B., Danjuma, M., Sama'ila, A., Salisu, B., Hafsatu, G. B.
Institution: Umaru Musa Yar'adua University
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4402771632