In vitro effect of the acidic pH on the susceptibility of the antimicrobial peptide Esc(1-21) against respiratory pathogens in cystic fibrosis lung environment
Year: 2024
Type: conference-paper
Abstract: Antimicrobial activity of Esc(1-21) against reference strains and clinical isolates of P. aeruginosaEsculentin(1-21) [Esc(1-21)] is a short-sized cationic antimicrobial peptide (AMP) corresponding to the N-terminal region of the frog-skin AMP esculentin-1a.It is endowed with a fast rate of killing activity, especially against Gram-negative bacteria, with a membrane perturbing mechanism of action.Background Esculentin-1a GIFSKLAGKKIKNLLISGLKNVGKEVGMDVVRTGIDIAGCKIKGEC GIFSKLAGKKIKNLLISGLKG-NH 2 +6 Basic residues Esc(1-21) ➢Short size ➢A large spectrum of activity (Gram -, Gram + , fungi) ➢A potent activity against planktonic and biofilm forms of Pseudomonas aeruginosa (Gram -).Rana esculenta [PEPTIDE] CAUSING 99,9% KILLING OF PLAKTONIC CELLS [PEPTIDE] CAUSING 95% KILLING OF BIOFILM CELLS 1 µM 12.5-25 µMPremise and aim of the workThe number of antibiotic-resistant microbial infections is dramatically increasing, while the discovery of new antibiotics is significantly declining.Furthermore, the activity of antibiotics is negatively influenced by the ability of bacteria to form sessile communities, called biofilms, and by the microenvironment of the infection, characterized by an acidic pH, especially in the lungs of patients suffering from cystic fibrosis (CF).AMPs represent interesting alternatives to conventional antibiotics, and with expanding properties.Here, we explored the effects of an acidic pH on the antimicrobial and antibiofilm activities of the AMP Esc(1-21).Notably, the activity of the peptide remained almost unchanged in the trend.These results highlight the potential of AMPs as novel antimicrobial agents, considering the enhanced resistance to antibiotics of the bacteria embedded in biofilms.
Source: European Peptide Symposium (EPS)
Institutions: Istituto Pasteur, Sapienza University of Rome
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4402846735