Year: 2024
Type: article
Abstract: A novel microfluidic ractopamine (RAC) detection platform consisting of a microfluidic RAC chip and a smart analysis device is proposed for the determination of RAC concentration in meat samples. This technology utilizes gold nanoparticles (AuNPs) modified with glutamic acid (GLU) and polyethyleneimine (PEI) to measure RAC concentration in food products. When RAC is present, AuNPs aggregate through hydrogen bonding, causing noticeable changes in their optical properties, which are detected using a self-built UV–visible micro-spectrophotometer. Within the range of 5 to 80 ppb, a linear relationship exists between the absorbance ratio (A693nm/A518nm) (Y) and RAC concentration (X), expressed as Y = 0.0054X + 0.4690, with a high coefficient of determination (R2 = 0.9943). This method exhibits a detection limit of 1.0 ppb and achieves results within 3 min. The practical utility of this microfluidic assay is exemplified through the evaluation of RAC concentrations in 50 commercially available meat samples. The variance between concentrations measured using this platform and those determined via liquid chromatography–tandem mass spectrometry (LC-MS/MS) is less than 8.33%. These results underscore the viability of the microfluidic detection platform as a rapid and cost-effective solution for ensuring food safety and regulatory compliance within the livestock industry.
Source: Biosensors
DOI: 10.3390/bios14100462
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,069
Awards: Microfluidic Point-Of-Care Detection Platform for Electrolytes Evaluation of Patients with Chronic Kidney Disease, Kidney Stones and Renal Function Diagnosis, Multifunctional Microfluidic Rapid Amplification and Detection System, Developing an Integrated Microfluidic Immunoassay Platform for Rapid Detection of Peritoneal Dialysate Biomarkers for Early Diagnosis and Prevention of Severe Peritoneal Sclerosis in Peritoneal Dialysis Patients ( II and II i)
OpenAlex ID: W4402859455