Work

Use of the senolytics dasatinib and quercetin for prevention of pelvic organ prolapse in a mouse animal model

Year: 2024

Type: article

Abstract: OBJECTIVE: mice, to assess the ability of dasatinib and quercetin (D+Q) to prevent development of prolapse. METHODS: (n=63) and wild-type (WT) mice (n=54) were assigned to control (vehicle injection) or treatment (D = 5 mg/kg, Q = 50 mg/kg) groups. Weekly oral gavage injections were administered from weeks 4-8 of life. Pelvic organ prolapse quantification system measurements were obtained weekly. Vaginal tissue was harvested at 10, 12 and 20 weeks. Tissue analysis included immunostaining for cell cycle inhibitors, multiplex cytokine analysis, senescence-associated-β-galactosidase (SA-β-Gal) and histologic analysis of extracellular matrix proteins. RESULTS: treatment mice increased compared to controls (1.04% vs. 0.84%, p=0.999). CONCLUSIONS: mice. This suggests senolytic agents may mitigate contributions of cellular senescence to tissue dysfunction associated with prolapse. Further studies are needed to confirm ideal timing, dosage, and route of senolytics in prevention of prolapse.

Source: Aging

DOI: 10.18632/aging.206120

Authors: Erryn Tappy, Haolin Shi, Jessica Pruszynski, Maria Florian-Rodriguez

Institution: The University of Texas Southwestern Medical Center

Language: English

Topic: Pelvic floor disorders treatments

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: University of Texas Southwestern Medical Center