Work

Rapid screening of Salmonella typhimurium based on a bacterial surface-imprinted polymer-functionalized bipolar electrode-electrochemiluminescence platform

Year: 2024

Type: article

Abstract: A bipolar electrode-electrochemiluminescence (BPE-ECL) sensor, utilizing a polydopamine (PDA) bacterial surface-imprinted polymer (SIP), has been developed for the detection of Salmonella typhimurium (S. typhimurium). To fabricate this sensor, dopamine (DA) served as the monomer, while S. typhimurium played a crucial role as the template molecule. Through a precision-tuned electropolymerization process, a polymer film template was seamlessly crafted on the cathode of BPE, encasing the target bacteria within its matrix. Once the template was delicately removed, a molecular surface-imprinted film emerged on the cathode, forming a PDA SIP capture probe that was remarkably adept at recognizing and binding to the target bacteria. When the target bacteria were present on the cathode, the Fc-aptamer-S. typhimurium acted as a signal probe to specifically recognized S. typhimurium. Applying a constant potential to the BPE, the oxidation product Fc+, generated at the cathode, accelerating the luminescence of the [Ru(bpy)3]2+/TPrA system on the anode. The sensor exhibited a remarkable sensitivity, capable of detecting S. typhimurium concentrations ranging from 101 to 106 CFU mL-1, with a detection limit of 1 CFU mL-1.

Source: Microchemical Journal

DOI: 10.1016/j.microc.2024.111788

Authors: Mengjuan Li, Yan Qi, Mingyao Xu, Fengyang Wang, Xiaohui Xiong, Yuanjian Liu

Institution: Nanjing Tech University

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,720