Work

Immunomodulatory peptides from sturgeon cartilage: Isolation, identification, molecular docking and effects on RAW264.7 cells

Year: 2024

Type: article

Abstract: Sturgeon cartilage is a by-product of the sturgeon caviar processing industry. Previous has been limited research in sturgeon by-products. This study aims to elucidate immunomodulatory peptides from sturgeon cartilage hydrolysates mechanisms. The sturgeon cartilage hydrolysate F2-7 displayed notable immunoreactivity. Additionally, western blotting confirmed that the immune enhancement attributed to F2-7 was mediated through promoting the expression of P-IKKα/β, IΚΚ, p65 and P-p65 proteins associated with the NF-κB signaling pathway. LC-MS/MS, molecular docking, and synthetic peptide experiments demonstrated that DHVPLPLP and HVPLPLP regulate the activity of the NF-κB signaling pathway by binding to IKK and p65 proteins through hydrogen bonding. Additionally, two peptides promote the proliferation of RAW264.7 macrophage cells, increase NO secretion, and enhance phagocytic activity. By activating macrophages and boosting immune system activity, their exhibit significant immunomodulatory capabilities, establishing their importance as key immunological peptides in the F2-7. DHVPLPLP and HVPLPLP are expected to be developed as novel immunomodulators.

Source: Food Chemistry X

DOI: 10.1016/j.fochx.2024.101863

Authors: Shuchan Li, Miaoqing An, Yuxuan Zhao, Wenjun Zhao, Pan Li, Bing Du

Institution: South China Agricultural University

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,350