Evaluation of Bartonella henselae IFA Seropositivity in Adult Patients Presenting with Various Symptoms at a Tertiary Education and Research Hospital
Year: 2024
Type: article
Study design: Observational Study
Abstract: Objective: Bartonella henselae, indirect , is the etiologic agent of cat scratch disease (CSD).Due to the difficulty of isolation and culture of Bartonella henselae, indirect fluorescent antibody (IFA) test is commonly used in diagnosis.In this study, we aimed to investigate the role of serology in the diagnosis of classical and atypical CSD by examining different serological titer values of patients with suspected CSD.Method: Patients were divided into 2 main groups as negative and positive according to the IFA test result.Patients with positive IFA titer were divided into 2 groups as suspected positive IFA (1:64-1:128) and positive IFA (≥1:256) and subgroup analysis was performed.Results: A total of 197 patients were included in the study and IFA tests were obtained from the laboratory database.The number of IFA immunoglobulin G seropositive patients was 57 (28.9%).The mean age of the patients was 37 (18-87) years, with 104 (52.8%) being female.While 49.7% of all patients had a history of cat contact, 61.4% of the patients in IFA test positive group had a history of cat contact (p=0.037).The most common symptom was lymphadenopathy (77.7%).Axillary lymphadenopathy was more common in the IFA positive group with a rate of 70.2% (p<0.001).The mean duration of LAP was 1 month in the IFA positive group and 2 months in the IFA negative group with a statistically significant difference (p<0.001).Unilateral LAP was significantly more common in the possible positive IFA group with a rate of 79.1% (p=0.043).Amaç: Bartonella henselae, kedi tırmığı hastalığının (KTH) etiyolojik etkenidir.Bartonella henselae'nin izolasyonu ve kültürünün zorluğu nedeniyle, tanıda dolaylı floresan antikor (İFA) testi yaygın olarak kullanılır.Bu çalışmada, KTH'den şüphelenilen hastaların farklı serolojik titre değerleri incelenerek, klasik ve atipik KTH'nin teşhisinde serolojinin rolü incelendi.Yöntem: Çalışmaya 01 Ocak 2018-01 Ocak 2024 tarihleri arasında KTH semptomları ile başvuran, 18 yaş üstü hastalar dahil edildi.Bartonella henselae İFA test sonucuna göre negatif ve pozitif olarak 2 gruba ayrıldı.İFA titresi pozitif hastalar, şüpheli İFA pozitif (1:64 ve 1:128) ve İFA pozitif (≥1:256) olarak 2 gruba ayrılıp alt grup analizi yapıldı.Bulgular: Laboratuvar veri tabanından toplam 197 hastada Bartonella henselae İFA testleri çalışıldığı saptandı.İFA immünoglobulin G seropozitif hasta sayısı 57 (%28,9) idi.Hastaların yaş ortalaması 37 (18-87) yıl ve 104 (%52,8) hasta kadındı.Tüm hastaların içinde kedi teması öyküsü %49,7'sinde mevcuttu.İFA test sonucu pozitif grubun %61,4'ünde kedi teması saptandı (p=0,037).En yaygın semptom lenfadenopatiydi (%77,7).İFA pozitif grupta aksiller lenfadenopati %70,2 ile daha fazlaydı (p<0,001).İFA pozitif grubun LAP ortalama süresi 1 ay, İFA negatif grubun ise 2 ay olup istatistiksel anlamlı farklılık saptandı (p<0,001).Pozitif İFA grubunda tek taraflı LAP %79, 1 oranla anlamlı düzeyde daha fazlaydı (p=0,043).
Source: Bagcilar Medical Bulletin
Authors: Burak Sarıkaya, Ayça Aydın
Institution: Sağlık Bilimleri Üniversitesi
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4402923220