Work

Protocol for the processing, cryopreservation, and biobanking of patient-derived cells and tissues

Year: 2024

Type: article

Abstract: Biobanking of patient-derived specimens offers unique opportunities for retrospective testing that could potentially contribute to diagnosing and evaluating clinical conditions, advancing personalized medicine and translational biomedical discovery. In this protocol, we detail the collection, processing, and cryopreservation of peripheral blood, bone marrow, and lymph nodes from patients with hematological malignancies. This protocol can be used for multiomics to gain cellular and molecular insights into blood cancers and to test the therapeutic potential of compounds for translational biomedical research. For complete details on the use and execution of this protocol, please refer to Lim et al. 1 and Rijal et al. 2 • Steps for processing and storage of peripheral blood, bone marrow, and lymph nodes • Details on retrieval of stored peripheral blood, bone marrow, and lymph nodes samples • Patient-derived samples can be used for multiomic applications and drug discovery Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Biobanking of patient-derived specimens offers unique opportunities for retrospective testing that could potentially contribute to diagnosing and evaluating clinical conditions, advancing personalized medicine and translational biomedical discovery. In this protocol, we detail the collection, processing, and cryopreservation of peripheral blood, bone marrow, and lymph nodes from patients with hematological malignancies. This protocol can be used for multiomics to gain cellular and molecular insights into blood cancers and to test the therapeutic potential of compounds for translational biomedical research.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103287

Authors: Abhimanu Pandey, Shweta Venkataraman, Si Ming Man, Dipti Talaulikar

Institutions: Australian National University, Act Health

Language: English

Topic: Hematopoietic Stem Cell Transplantation

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500