Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection
Year: 2024
Type: article
Abstract: Fig. 1: Schematic illustration of the principle of the Dual targets driven SPEXPAR assisted MNAzyme (D-S-M) fluorescent biosensor for olfactory marker protein (OMP) detection. A. Workflow for detection of OMP in nasal swab. B. Isothermal Self-Primer EXPonential Amplification Reaction (SPEXPAR) amplification. C. The production of fluorescent signal by Multicomponent Nuclear Acid Enzyme (MNAzyme). The signal of OMP was amplified and changed into the detectable fluorescence signal based on the reactions of SPEXPAR and MNAzyme in the D-S-M fluorescence biosensor. The qualitative or quantitative analysis of OMP can be measured by the analysis of the fluorescence intensity.Image 1.
Source: Materials Today Bio
Authors: Jing Qi, Xuemin Cao, Hongyi Bao, Tuodi Zhang, Yichen Wang, Ya Wen, Junling Yang, Guixuan Ge, Ping Wang, Lin Chen, Feng Wang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,150
Awards: G7714750963, G8301122748
OpenAlex ID: W4402932245