Fluorogenic cell surface labelling using fluorescent molecular rotor-labelled peptide boronic acid conjugates
Year: 2024
Type: conference-paper
Abstract: Abnormally viscous mucus hydrogel layers represent the key pathological characteristic of chronic, muco-obstructive respiratory and gastrointestinal disorders such as Cystic Fibrosis (CF), Chronic Obstructive Pulmonary Disease (COPD) and Inflammatory Bowel Disease (IBD).To date, quantifying and mapping spatial nanoviscosity on living cells/tissues poses a major challenge.Our aim is to probe viscosity by anchoring fluorescent molecular rotor (FMR) dyes to cell surface glycans.For this purpose, the FMRs are attached to peptide-multiboronic acid conjugates.We show the development of fluorogenic probes that have high affinity for cell surfaces and demonstrate ratiometric imaging with an FMR and a non-FMR probe to characterize cell surface nanoviscosities.
Source: European Peptide Symposium (EPS)
Authors: Alen Koçak, Pritam Ghosh, Oliver Seitz
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4402942205