Development and Validation of Analytical Method for Concurrent Determination of Mupirocin and Beclomethasone Dipropionate in Pharmaceutical Formulation and Perform Forced Degradation Study
Year: 2024
Type: article
Abstract: Simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of both drugs in their combined dosage form.In RP-HPLC, analysis is carried out using Methanol: Buffer pH 4 (65:35, v/v) mobile phase and Phenomenex Gemini ODS C18 column (200 mm x 4.6 mm, 5.0 µ particle size) as stationary phase with detection wavelength of 215 nm.Linearity was obtained in the concentration range of 40-120 µg/ml and 0.5-1.5 µg/ml for Mupirocin and Beclomethasone dipropionate respectively.The % recoveries of the both the drugs were found to be 99.45-99.86%and 100.10-100.39%respectively.LOQ were found to be 29.11µg/ml and 0.297µg/ml at 215 nm for Mupirocin and Beclomethasone dipropionate respectively.Methods were statistically validated for accuracy, precision, specificity, LOQ and robustness.Also carried out a Force degradation study according to ICH guidelines and can be used for analysis of combined dosage form.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4402991293